Protein Expression Facility

The Protein Expression Facility is an integrated partner of the Biochemistry and Biophysics Center, dedicated to all things protein-related. Its mission is to offer assistance to its members, as well as outside collaborators, in the form of, but not restricted to: custom plasmid design, large scale fermentation, protein purification, biochemical characterization, and biophysical quality control.

The overall philosophy of the Protein Expression Facility is to provide researchers with knowledge and expertise, tools for their work, as well as guidance and mentoring in an all-inclusive and participatory manner.

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Nico Tjandra

Featured Publications

Structural basis for polyglutamate chain initiation and elongation by TTLL family enzymes.

Authors: Mahalingan KK, Keith Keenan E, Strickland M, Li Y, Liu Y, Ball HL, Tanner ME, Tjandra N, Roll-Mecak A.
Nat Struct Mol Biol. 2020 Sep 01;27(9):802-813
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Squeezing lipids: NMR characterization of lipoprotein particles under pressure

Authors: Starich MR, Tang J, Remaley AT, Tjandra N.
Chem Phys Lipids 2020 May 01;228:228
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RNA Binding Suppresses Tsg101 Recognition of Ub-Modified Gag and Facilitates Recruitment to the Plasma Membrane

Authors: Watanabe SM, Strickland M, Tjandra N, Carter CA.
Viruses 2020 Apr 15;12(4):447
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Selective Targeting of Virus Replication by Proton Pump Inhibitors.

Authors: Tjandra N, Carter C.
Sci Rep. 2020 Mar 04;10(1):4003
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Bax expression is optimal at low oxygen tension and constant agitation.

Authors: He Y, Chen Y, Morris DL, Lee DY, Tjandra N.
Protein Expr Purif. 2020 Jan 01;165:105501
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Meet the Team

Nico Tjandra

Nico Tjandra, Ph.D.

Senior Investigator

Nico Tjandra graduated with a M.S. in physics and Ph.D. in physics/biophysics from Carnegie Mellon University. He was a National Research Service Awards postdoctoral fellow at the National Institute of Diabetes, Digestive, and Kidney Diseases (NIDDK) at the NIH. He became a tenure-track Investigator in 1997 and was promoted to Senior Investigator in the Structural Biophysics Section in 2001. He received the International Council of Magnetic Resonance in Biological Systems Founder’s Medal in 2005. Dr. Tjandra has authored or coauthored more than 150 papers and book chapters. He has also organized a hands on summer course at the NIH, “Structure Determination of Biological Macromolecules by Solution NMR,” designed for extramural Ph.D. students who could benefit from learning new methodologies in NMR for their thesis work. He is one of the long-time instructors in the EMBO practical course on studying biomacromolecules by NMR.

Contact the lab

50 South Dr
Bethesda, MD 20814
United States
Phone:
301-402-3029
Fax:
301-402-3405