Graphical Abstracts
Ramos, S. & Lee, J.C. (2024) Raman spectroscopy in the study of amyloid formation and phase separation, Biochem. Soc. Trans. 52 1121-1130. DOI: 10.1042/BST2023055
Shadish, J.R. & Lee, J.C. (2024) Genetically encoded lysine photocage for spatiotemporal control of TDP-43 nuclear import, Biophys. Chem. 307, 107191. DOI: 10.1016/j.bpc.2024.107191
Ramos, S. & Lee, J.C. (2023) Water bend‒libration as a cellular Raman imaging probe of hydration, Proc. Natl. Acad. Sci. U.S.A., 120, e2313133120. DOI: 10.1073/pnas.2313133120
Watson, M.D. & Lee, J.C. (2023) Genetically encoded aryl alkyne for Raman imaging of intracellular α-synuclein fibrils, J. Mol. Biol. 435, 167716. DOI: 10.1016/j.jmb.2022.167716
Shuster, S.O. & Lee, J.C. (2021) Tryptophan probes of TDP-43 C-terminal domain amyloid formation, J. Phys. Chem. B., 125, 3781-3789. DOI: 10.1021/acs.jpcb.1c00767
Kaur U. & Lee, J.C. (2021) Membrane interactions of α-synuclein probed by neutrons and photons, Acc. Chem. Res. 54, 302-310. DOI: 10.1021/acs.accounts.0c00453
Watson, M.D., Flynn, J.D., Lee, J.C. (2021) Raman spectral imaging of 13C2H15N-labeled α-synuclein amyloid fibrils in cells, Biophys. Chem. 269, 106528. DOI: 10.1016/j.bpc.2020.106528
Flynn, J.D., Gimmen, M., Dean, D.N., Lacy, S.M., Lee, J.C. (2020) Terminal alkynes as Raman probes of α-synuclein in solution and in cells, ChemBioChem, 21, 1582-1586. DOI: 10.1002/cbic.202000026
Watson, M.D. & Lee, J.C. (2019) N-terminal acetylation affects α-synuclein fibril polymorphism, Biochemistry, 58, 3630-3633. DOI: 10.1021/acs.biochem.9b00629
Ni, X., McGlinchey, R.P., Jiang, J., Lee, J.C. (2019) Structural insights into α-synuclein fibril polymorphism: Effects of Parkinson’s disease-related C-terminal truncations, J. Mol. Biol. 431, 3913-3919. DOI: 10.1016/j.jmb.2019.07.001
Flynn, J.D., Jiang, Z., Lee, J.C. (2018) Segmental 13C-labeling and Raman microspectroscopy of α-synuclein amyloid formation, Angew. Chem. Int. Ed. Engl. 57, 17069-17072. DOI: 10.1002/anie.201809865
Flynn, J.D. & Lee, J.C. (2018) Raman fingerprints of amyloid structures, Chem. Commun. 54, 6983-6986. DOI: 10.1039/c8cc03217c
Jiang, Z., Flynn, J.D., Teague, W.E., Gawrisch, K., Lee, J.C. (2018) Stimulation of α-synuclein amyloid formation by phosphatidyglycerol micellar tubules, Biochim. Biophys. Acta Biomembr.1860, 1840-1847. DOI:10.1016/j.bbamem.2018.02.025
Flynn, J.D., McGlinchey, R.P., Walker III, R.L., Lee, J.C. (2018) Structural features of α-synuclein amyloid fibrils revealed by Raman spectroscopy, J. Biol. Chem. 293, 767-776. DOI:10.1074/jbc.M117.812388
McGlinchey, R.P., Dominah, G., Lee, J.C. (2017) Taking a bite out of amyloid: Mechanistic insights into α-synuclein degradation by cathepsin L, Biochemistry, 56, 3881-3884. DOI: 10.1021/acs.biochem.7b00360
Jiang, Z., Heinrich, F., McGlinchey, R.P., Gruschus, J.M., Lee, J.C. (2017) Segmental deuteration of α-synuclein for neutron reflectometry on tethered bilayers, J. Phys. Chem. Lett. 8, 29-34. DOI: 10.1021/acs.jpclett.6b02304
Brisbois, C.A. & Lee, J.C. (2016) Apolipoprotein C-III nanodiscs studied by site-specific tryptophan fluorescence, Biochemistry, 55, 4939-4948. DOI: 10.1021/acs.biochem.6b00599
de Messieres M., Ng A., Duarte, C.J., Remaley, A.T., Lee, J.C. (2016) Single particle-tracking of human lipoproteins, Anal. Chem. 88, 596-599. DOI: 10.1021/acs.analchem.5b03749
Pfefferkorn, C.M., Walker III, R.L., He, Y., Lee, J.C. (2015) Tryptophan probes reveal residue-specific phospholipid interactions of apolipoprotein C-III, Biochim. Biophys. Acta Biomembr.,1848, 2821-2828. DOI: 10.1016/j.bbamem.2015.08.018
Jiang, Z., Hess, S.K., Heinrich, F., Lee, J.C. (2015) Molecular details of α-synuclein membrane association probed by neutrons and photons, J. Phys. Chem. B 119, 4812-4823. DOI: 10.1021/jp512499r
Yap, T.L., Jiang, Z., Heinrich, F., Gruschus, J.M., Pfefferkorn, C.M., Barros M., Curtis, J.E., Sidransky, E., Lee, J.C. (2015) Structural features of membrane-bound glucocerebrosidase and α-synuclein probed by neutron reflectometry and fluorescence spectroscopy, J. Biol. Chem. 290, 744-754. DOI: 10.1074/jbc.M114.610584
Jiang, Z. & Lee, J.C. (2014) Lysophospholipid-containing membranes modulate the fibril formation of the repeat domain of a human functional amyloid, Pmel17, J. Mol. Biol. 426, 4074-4086. DOI: 10.1016/j.jmb.2014.10.009
McGlinchey, R.P., Jiang, Z., Lee, J.C. (2014) Molecular origin of the pH dependent fibril formation of a functional amyloid, ChemBioChem, 15, 1569-1572. DOI: 10.1002/cbic.201402074
Jiang, Z., de Messieres, M., Lee, J.C. (2013) Membrane remodeling by α-synuclein and effects on amyloid formation, J. Am. Chem. Soc. 135, 15970-15973. DOI: 10.1021/ja405993r
Yap, T.L., Gruschus, J.M., Velayati, A., Sidransky, E., Lee, J.C. (2013) Saposin C protects glucocerebrosidase against α-synuclein inhibition, Biochemistry 52, 7161-7163. DOI: 10.1021/bi401191v
Gruschus, J.M., Yap, T.L., Pistolesi, S., Maltsev, A.S., Lee, J.C. (2013) NMR structure of calmodulin complexed to an N-terminally acetylated α-synuclein peptide, Biochemistry 52, 3436-3445.
McGlinchey, R.P., Gruschus, J.M., Nagy, A., Lee, J.C. (2011) Probing fibril dissolution of the repeat domain of a functional amyloid, Pmel17, on the microscopic and residue level, Biochemistry 50, 10567-10569. DOI: 10.1021/bi201578h
Yap, T.L., Pfefferkorn, C.M., Lee, J.C. (2011) Residue-specific fluorescent probes of α-synuclein: Detection of early events at the N- and C-termini during fibril assembly, Biochemistry 50, 1963-1965. DOI: 10.1021/bi2000824
Lucas, H.R., DeBeer, S., Hong, M.-S., Lee, J.C. (2010) Evidence for copper-dioxygen reactivity during α-synuclein fibril formation, J. Am. Chem. Soc. 132, 6636-6637.DOI: 10.1021/ja101756m
Pfefferkorn, C.M. & Lee, J.C. (2010) Tryptophan probes at the α-synuclein and membrane interface, J. Phys. Chem. B. 114, 4615-4622. DOI: 10.1021/jp908092e
Meet the Team
Jennifer Lee, Ph.D.
Jennifer C. Lee graduated with a B.S. in chemistry and a B.A. in economics from the University of California, Berkeley, in 1997, and earned her Ph.D. in chemistry from the California Institute of Technology in 2002. Following a one-year postdoctoral stint at the University of Southern California, she became a Beckman Senior Research Fellow at the Beckman Institute Laser Resource Center at the California Institute of Technology where she investigated the structures and dynamics of an intrinsically disordered and amyloid forming protein using time-resolved spectroscopic measurements. In 2006, Dr. Lee joined the NHLBI as a tenure-track Investigtor. She was awarded an NIH Graduate Partnerships Program Outstanding Mentor Award in 2009. Dr. Lee is a member of the American Chemical Society and the Protein Society.
Contact the lab
Matthew Watson, Ph.D.
Ryan McGlinchey, Ph.D.
Sashary Ramos, Ph.D.
Daniel SanGiovanni
Tai Vu
Kevin Gery
Join Our Lab
Postdoctoral Fellowship
In expanding the scope of our research, a candidate with strong backgrounds in cell biology and fluorescence microscopy, including…