Flow Cytometry Core

The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting, cell analysis and Immune monitoring needs. A wide variety of cytometry applications are supported from all types of samples originating from Human, Mouse, Rat, NHP, Drosophila, etc.

1. Assisted Service Facility (Bldg. 10 section: 8th floor, Room 8C104)
   • State-of-the-art aseptic sorting under BSL-2 conditions
     • 1-to-6-way sorting of cells, nuclei, extracellular vesicles, chromosomes.
     • Low-pressure, gentle sorting for cell cloning, iPSC, and genomics studies (Including single cell RNA Seq and 10X Genomics).
     • High-speed bulk and single cell sorting for functional and genomics studies.
   • High parameter cell analyses (up to 40 colors)
     • Panel development and implementation for immunophenotyping studies.
     • One-on-one personal assistance for methods development and standardizing protocols for sample preparation, tissue dissociation and deep immunophenotyping. We also support studies investigating apoptosis, cell cycle and ploidy, cell proliferation, drug screening, CRISPR screening/genotyping, and kinetics analysis, such as calcium flux.
   • State-of-the-art image cytometry (10 colors; up to 60X magnification)
     • Analyses of nuclear translocation, cellular colocalization, and cell-to-cell interactions.
   • Multiplexed quantitation of soluble analytes (Luminex platform)
     • Bead-based immune analyses of blood and body fluids, cell lysates, and culture supernatants.
   • Training and Consultation
     • One-on-one personal training for instrument operation, methods development, and troubleshooting.
     • Teaching of best flow cytometry practices for data generation and analysis.
     • Perform extended data analyses as needed.
2. 24 x 7 Self-service Facility
   • 24 x 7 operation at Bldg. 10 8th floor Corridor C (8C104), for all analyzers and at CRC 3rd floor (Rooms 3-5217, 3-5225, and 3-5209) for all analyzers and the Melody cell sorter by trained users.
   • Assisted aseptic cell sorting, high parameter cell analyses (up to 40 colors), one-on-one assay specific panel design, methods development, training and teaching best practices of flow cytometry for data analysis services are also available on request using Melody cell sorter, and cell analyzers in CRC 24 x 7 section.

Cytometers

• Cell Sorters: (Single cell or bulk; 1 to 6 way)
  • 40 color 5 Laser 6-way BSL rated BD Discover S8 with cell view spectral sorter.@
  • 30 color 6 Laser 6-way BSL rated BD S6. @
  • 17 color 5 Laser 4-way BSL rated BD Fusion. @
  • 17 color 5 Laser 4-way SORP BD Aria-IIu. @
  • 12 color 3 Laser 4-way BSL rated BD Melody. #
• Cell Analyzers:
  • 40 color 5 Laser Cytek Aurora spectral analyzer with HTS. #
  • 30 color 7 Laser BD Symphony A5 with HTS. @
  • 19 color 5 Laser BD LSR Fortessa with HTS. @
  • 17 color 4 Laser BD LSR Fortessa‐1 with HTS. #
  • 19 color 5 Laser BD LSR Fortessa‐2 with HTS. #
  • 9 color BD Canto‐II. #
  • 7 color BD Canto-I. #
• Imaging Flow Cytometer:
  • 5 Laser, 10 colors Amnis Imagestream Gen-X Mark II Analyzer with HTS. @
• Immunoassays:
  • Luminex 200 system. @
• Workstation for post-acquisition data analysis equipped with FlowJoTM (+ plugins for advance analysis), SpectroFloTM, IDEAS 6.2TM, R, MS Office.
  • Workstation-1 @
  • Workstation-2 #

@ Location: Bldg.10, 8th floor, RN 8C104.
# Location: Bldg.10, CRC 3rd floor, Rooms 3-5217, 3-5225, and 3-5209.